Trypsin MP Biomedical Hàng Có Sẵn

SKU

02101179-CF

Application Notes

Trypsin can be used to dissolve blood clots in its microbial form and treat inflammation in its pancreatic form. Trypsin is commonly used in biological research.

Biochemical Physiological Actions

Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity.

Components

Trypsin is composed of two subunits, α-trypsin and β-trypsin. α-Trypsin is composed of two peptide chains and β-trypsin is composed of one chain.

EC Number

232-650-8

Extinction Coefficient

E1%280 = 14.3 (Lit.)

Format

lyophilized powder

Grade

2× Crystallized

Hazard Statements

H315-H319-H334-H335

Isoelectric Point

pH 10.5(Lit.)

Applications

Dissolve blood clots

Personal Protective Equipment

Dust mask , Eyeshields, Faceshields, Gloves

Physical Appearance

White Powder

Product Families Description

Trypsin

Product Overview

Trypsin

RTECS Number

YN5075000

Safety Symbol

GHS07, GHS08

Source

Beef pancreas

Specific Activity

≥180 TAME u/mg

Specificity

The protease activity of trypsin is highly specific toward positively charged side chains with lysine and arginine. Forms complexes with a2-macroglobulin. Can be used in the isolation of intact; detergent-free phycobilisomes and in the hydrolysis/condensation of carboxylic ester bonds.

Storage and Handling

+4 °C

Typical Working Concentration

For the Removal of Adherent Cells from a Culture Surface: 2.5% w/v solution

Unit Definition

One unit hydrolyzes one µmole of TAME per minute at 25 °C, pH 8.2, in the presence of ionized calcium.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.